Detecting Antibodies With Glowing Proteins, Thread and a Smartphone

Light emitted from sensor proteins turned bluer when samples contained higher concentrations of antibodies against three viruses. Adapted from ACS Sensors 2020, DOI: 10.1021/acssensors.0c00564

To defend the body, the immune system makes proteins known as antibodies that latch onto the perceived threat, be it HIV, the new coronavirus or, as is the case in autoimmune disease, part of the body itself. In a new proof-of-concept study in ACS Sensors, researchers describe a new system for detecting antibodies within a pinprick of blood within minutes, using an unlikely combination of cotton thread, glowing proteins and a smartphone camera.

While some tests simply detect the presence of an antibody, sometimes doctors want to know how much is circulating in the blood. Such quantitative tests are used to diagnose a number of conditions, including infections and autoimmune diseases. Although a quantitative antibody test is not yet approved for use in the U.S., such a test could potentially aid in assessing immunity to SARS-CoV-2. However, quantitative testing currently requires expensive, sophisticated instruments in labs, and efforts to make it more accessible have had only limited success. So, Maarten Merkx, Daniel Citterio and colleagues tested an approach that could provide a small, inexpensive alternative.

The researchers' microfluidic thread-based analytical device (μTAD) relies on light-emitting sensor proteins held on a thread. In the presence of the right antibodies, the color of the light emitted by the sensors changes. The shift, from green to blue, correlates with the concentration of antibodies in a sample. Using a finger-prick-sized drop of pigs' blood spiked with antibodies against HIV, the team showed that their system could successfully detect antibody levels within five minutes. In addition, the device can test for the amounts of several different antibodies in a single blood sample and doesn't require extensive handling and incubation steps. They found that a smartphone camera, outfitted with an adaptor, could pick up on the shifts in the light's color, while the device itself could convert color data into test results and transmit that information. With further development, this combination of technologies could provide user-friendly, one-step analysis of antibody concentration, according to the researchers.

The authors acknowledge funding from the Japan Society for the Promotion of Science through a Bilateral Joint Research Projects/Seminars grant and a Grant-in-aid for Scientific Research, the Terumo Life Science Foundation, and the European Research Council Starting and Proof of Concept grants.

Source: American Chemical Society (ACS)

53 Comments on "Detecting Antibodies With Glowing Proteins, Thread and a Smartphone"

  1. Way cool! Some extremely valid points! I appreciate you writing this post
    and the rest of the site is also very good.

  2. I know this site provides quality depending articles and additional
    stuff, is there any other website which gives these kinds
    of information in quality?

  3. I will immediately take hold of your rss feed as I can’t to find your email subscription link
    or newsletter service. Do you have any? Please let me understand in order
    that I may subscribe. Thanks.

  4. Great article. I will be going through many of these issues as well..

  5. Hello, I enjoy interpretation through and through your post.
    I similar to spell a piddling scuttlebutt to put up you.

  6. Jolly constituent of cognitive content. I just stumbled
    upon your web site and in accession capital letter
    to exact that I get actually enjoyed answer for
    your web log posts.

  7. Smear on with this write-up, I utterly believe that this site inevitably far Thomas More care.

  8. It’s identical gentle to breakthrough come
    out whatever weigh on nett as compared to books, as I ground
    this piece of committal to writing at this WWW

  9. Jolly chemical element of content. I but stumbled upon your website and in entree cap to
    take that I develop in reality enjoyed report your web log posts.

  10. Highly energetic article, I liked that bit. Will there be a part 2?

  11. You could unquestionably go through your exuberance within the solve you publish.

  12. you spend a penny blogging take care lenient. The boilers suit looking of your entanglement
    situation is excellent, as good as the contentedness!

  13. It’s selfsame loose to encounter come out whatever issue on meshing as compared to books, as I establish this
    patch of composition at this World Wide
    Web Page.

  14. Hello, I delight indication through your spot.
    I the like to publish a trivial gossip to back you.

  15. What host are you exploitation? Buns I contract your associate connect to your emcee?

  16. Excellent, what a web log it is! This internet site provides
    utile facts to us, living it up.

  17. You could emphatically go through your enthusiasm within the ferment you drop a line.

  18. Pretty component of depicted object. I simply stumbled upon your situation and
    in admittance capital letter to arrogate that I acquire in reality enjoyed report your blog posts.

  19. Hi, Neat post. There is an issue together with your website in web
    explorer, may test this? IE still is the marketplace chief and a huge section of other people
    will pass over your excellent writing because of this problem.

  20. Fantastic post however I was wanting to know if you could write a litte more on this subject?

    I’d be very thankful if you could elaborate a little bit more.

  21. Hello, yup this post is really fastidious and I have learned lot of things from it on the topic of blogging.

  22. It’s nearly impossible to find knowledgeable people
    for this subject, but you sound like you know what you’re talking
    about! Thanks

  23. Great article! That is the type of information that should be shared around the internet.

    Disgrace on the search engines for no longer positioning this publish upper!
    Come on over and consult with my site . Thank you =)

  24. I pay a quick visit each day some web pages and sites to read posts, except this website provides quality
    based posts.

  25. Very nice article, just what I was looking for.

  26. Hello there! Do you use Twitter? I’d like
    to follow you if that would be ok. I’m definitely enjoying your blog and look forward to new updates.

  27. I was recommended this web site by my cousin. I am not sure whether this post is written by him as nobody else know such detailed about my trouble. You are amazing! Thanks!kindly join us.
    Take a look at my homepage –

  28. I simply want to say I am newbie to blogs and truly loved this blog. Likely I’m likely to bookmark your website . You surely have incredible posts. Thanks a bunch for sharing with us your webpage.Please join us.

  29. Hi there, I found your blog by the use of Google whilst searching for a comparable
    topic, your site came up, it seems to be great. I’ve bookmarked it in my google bookmarks.

    Hello there, just became aware of your weblog via Google, and found that it is really informative.
    I am going to watch out for brussels. I’ll be grateful
    if you continue this in future. Numerous people can be benefited
    from your writing. Cheers!

Leave a comment

Your email address will not be published.